We present a fast, reliable and inexpensive restriction-free 30x24x24 wall cabinet cloning method for seamless DNA insertion into any plasmid without sequence limitation.Exponential megapriming PCR (EMP) cloning requires two consecutive PCR steps and can be carried out in one day.We show that EMP cloning has a higher efficiency than restriction-fre
ABSTRACT Trypanosoma brucei gambiense is the primary causative agent of human African trypanosomiasis (HAT), a vector-borne disease endemic to West and Central Africa.The extracellular parasite evades antibody recognition within the host bloodstream by altering its variant surface glycoprotein (VSG) coat through a process of antigenic variation.The
Osteoarthritis (OA) is a leading cause of pain and disability which results in a reduced quality of life.Due to the avascular nature of cartilage, damaged cartilage has a finite capacity for healing or regeneration.To date, conservative management, including physical measures and pharmacological therapy are still the quest fryer principal choices o
ABSTRACT Introduction: Ischemia-reperfusion (IR) injury results from inflammation and oxidative stress, among other factors.Because of its anti-inflammatory and antioxidant properties, the Brazil nut (BN) might attenuate IR renal injury.Objective: The aim of the present study was to investigate whether the intake of BN prevents or reduces IR kidney