Exponential megapriming PCR (EMP) cloning--seamless DNA insertion into any target plasmid without sequence constraints.

Exponential megapriming PCR (EMP) cloning--seamless DNA insertion into any target plasmid without sequence constraints.

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We present a fast, reliable and inexpensive restriction-free 30x24x24 wall cabinet cloning method for seamless DNA insertion into any plasmid without sequence limitation.Exponential megapriming PCR (EMP) cloning requires two consecutive PCR steps and can be carried out in one day.We show that EMP cloning has a higher efficiency than restriction-free (RF) cloning, ribavirin coupon especially for long inserts above 2.5 kb.

EMP further enables simultaneous cloning of multiple inserts.